A Population Genetics Study of Anopheles Darlingi (Diptera: Culicidae) from Colombia Based on Random Amplified Polymorphic DNA-Polymerase Chain Reaction and Amplified Fragment Length Polymorphism Markers
Abstract
The genetic variation and population structure of three populations of Anopheles darlingi from Colombia were studied using random amplified polymorphic markers (RAPDs) and amplified fragment length polymorphism markers (AFLPs). Six RAPD primers produced 46 polymorphic fragments, while two AFLP primer combinations produced 197 polymorphic fragments from 71 DNA samples. Both of the evaluated generic markers showed the presence of gene flow, suggesting that Colombian An. darlingi populations are in panmixia. Average genetic diversity, estimated from observed heterozygosity, was 0.374 (RAPD) and 0.309 (AFLP). RAPD and AFLP markers showed little evidence of geographic separation between eastern and western populations; however, the FsubST valued showed high gene flow between the two western populations (RAPD: FsubST = 0.029; Nm: 8.5; AFLP: FsubST = 0.051; Nm: 4.7). According to molecular variance analysis (AMOVA), the genetic distance between populations was significant (RAPD: PhisubST = 0.084: AFLP: PhisubST = 0.229, P < 0.001). The FsubST distances and AMOVAs using AFLP loci support the differentiation of the Guyana biogeographic province population from those of the Choco-Magdalena. In this last region, Choco and Cordoba populations showed the highest genetic flow.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2007
- Accession Number
- ADA503853
Entities
People
- Carmen E. Posso
- Felipe Garcia
- Gerardo Gallego
- Heiber Cardenas
- Marco F. Suarez
- Myriam C. Duque
- Ranulfo Gonzalez
- Richard Wilkerson
Organizations
- Walter Reed Army Institute of Research