Development of a Conditional Transgenic Mouse Model to Test the Fallopian Tube Origin of Serous Ovarian Cancer
Abstract
The purpose of this research study is to generate transgenic mice to specifically address fallopian tube tumorigenesis and test the potential for tubal origin of high-grade serous ovarian cancer observed in patient samples. To this end, we have generated two independent lines of transgenic reporter gene mice using the mouse oviduct-specific glycoprotein-1 (Ovgp1) promoter driving expression of the bacterial beta-galactosidase (lacZ) gene. Transgene expression is readily observed in the oviduct of female mice and is concomitant with the onset of puberty. Expression is specifically localized to the oviductal epithelium lining the lumen. In the highest expressing Ovgp1-lacZ transgenic line, however, expression is also significant in the stroma of the uterus. Ongoing studies are focused on understanding the regulation of transgene expression to develop a better method to restrict expression to the oviduct epithelium specifically. We believe our results will lay the foundation to generate an accurate mouse model to study the tubal origin of serous ovarian cancer in humans.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2009
- Accession Number
- ADA504112
Entities
People
- Trevor Shepherd
Organizations
- Western University