Signature and Mechanism of the Epithelial-to-Mesenchymal Transition

Abstract

The Epithelial-to-Mesenchymal Transition (EMT) is a conserved developmental process that is thought to be reactivated during the metastasis of epithelial cancers such as breast cancer. This study seeks to identify genes commonly regulated in the EMT, and identify key regulators of the process. From the successful candidate regulators, a core genetic circuit that controls the EMT will be constructed. The relevance of this circuit, or portions of it, to cancer metastasis, particularly in breast cancer models, will be investigated. An EMT core gene signature of approximately 1000 genes was generated, from which the transcription factor Zeb1 emerged as a potential key regulator of the EMT. Zeb1 demonstrated a much stronger EMT-inducing ability than previously known factors. The relationship between known EMT-inducing factors has not previously been studied, so this represents a novel finding. Efforts are underway to construct the genetic circuit stemming from Zeb1.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2009
Accession Number
ADA504655

Entities

People

  • Kong J. Kah

Organizations

  • Massachusetts Institute of Technology

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Diseases And Disorders
  • Epithelial Cells
  • Gene Expression
  • Genes
  • Genetics
  • Massachusetts
  • Metastasis
  • Neoplasms
  • Regulators
  • Transcription Factors
  • Transitions
  • United States

Readers

  • Molecular and genetic basis of cancer.
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology