The Role of ABC Proteins in Drug Resistant Breast Cancer Cells

Abstract

This predoctoral training grant, supported by the Department of Defense Breast Cancer Research Program (BCRP) of the Office of the Congressionally Directed Medical Research Programs (CDMRP), aims to study the possible role of membrane transporters in pleitropic drug resistance, using a combination of molecular biological, biochemical, and biophysical methods. The third year of funding was spent in further characterization of the Plasmodium falciparum Chloroquine Transporter (PfCRT), a member of a drug/metabolite transporter superfamily. Data from the previous year provided proof of the protein's direct interaction with its drug substrate. I have subsequently employed a methodology commonly applied to drug resistance proteins (such as human P-glycoprotein) and defined the drug binding site through mass spectrometry. This report summarizes work conducted from March 2008 through June 2008, when my work on this project ceased due to graduation. A request for a change of PI for this award has been submitted and is pending review.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2009
Accession Number
ADA504701

Entities

People

  • Jacqueline K. Lekostaj
  • Perri Pleeter

Organizations

  • Georgetown University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Chemistry
  • Department Of Defense
  • Drug Resistance
  • Malaria
  • Mass Spectra
  • Mass Spectrometry
  • Membranes
  • Neoplasms
  • Peptides
  • Proteins
  • Resistance
  • Spectrometry
  • Training

Fields of Study

  • Biology
  • Chemistry

Readers

  • Clinical Trial Research.
  • Molecular and Cellular Biochemistry
  • Parasitology and Pharmacology of Malaria.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech