Discovery of Novel Metastasis Genes in Breast Cancer
Abstract
Under this proposal we sought to identify genes that drive the homing of invasive primary breast cancer to its metastatic sites in the brain, lungs and bone marrow. We used phage display cDNA libraries generated from human breast cancer cells for in vivo selection from targeted organs. Ten distinct genes were identified. For the different sites, no overlap between the genes identified was observed. The genes identified were not known to contribute to breast cancer metastasis. We found mRNA expression in archival, clinical samples of formalin-fixed, paraffin-embedded in vasive breast cancer tissues by in situ hybridization. Functional studies that use the invasion of breast cancer cells into endothelial monolayers are used to evaluate the contribution of these newly identified metastasis genes to breast cancer cell attachment to endothelial cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2005
- Accession Number
- ADA505021
Entities
People
- Anton Wellstein
Organizations
- Georgetown University