Identification of Mechanisms of Breast Cancer Metastasis Using Tissue Specific Virus Delivery
Abstract
In the first year of support we established protocols for efficient in vitro cultivation and infection of mammary gland derived normal, hyperplastic and tumorigenic cells. For the second year we reported the analyses of mammary tumor cells when infected with candidate genes that are likely to promote invasion. We established a three dimensional basement membrane based cell culture system for primary mouse mammary cells that will allow us to examine differences in normal, hyperplasic, tumorigenic and invasive behavior of infected mammary cells in more detail. In the last funding period we studied the effect of inducible oncogenes on established polarized acini. We monitored oncogenic growth, tumor maintenance and cell autonomous tumor regression of primary mammary cells. We managed to identify a special subset of cells, located at the rim of the structures, that was protected from cell death after oncogene withdrawal. This outer layer of cells was capable of re-initiating tumorigenic growth upon renewed doxycycline exposure and may be a surrogate of dormant tumor cells, the potential substrate of tumor relapse.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2008
- Accession Number
- ADA505043
Entities
People
- Martin Jechlinger
Organizations
- Memorial Sloan Kettering Cancer Center