Nanothermite-Based Microsystem for Drug Delivery and Cell Transfection
Abstract
This paper describes a new system for pressure-induced cell transfection. The system generates pressure pulses from a microchip that contains a small quantity of nanothermite material and an electrical igniter. The pressure output from the nanothermite reaction is coupled to the biological target through a PDMS membrane and a tube filled with gelatin. The system generates pressure pulses in the range of 10-40MPa. It has been used to transfect primary cells with 99% transfection rate and cell survival. It has also been used to transfect cell lines (Hela, HL-60, and HT-29). In all cases survival was >99%, and transfection rate in Hela, HL60 and HT-29 was up to 37 60 and 30% respectively. In addition, the system has been shown to transfect intact spinal cords and arteries from chicken (St 30) with no noticeable damage.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2008
- Accession Number
- ADA505079
Entities
People
- A. Bezmelnitsyn
- Keshab Gangopadhyay
- L. Polo-parada
- R. Thiruvengadathan
- S. Apperson
- S. Gangopadhyay