Molecular Confirmation of the Specific Status of Anopheles halophylus (Diptera: Culicidae) and Evidence of a New Cryptic Species within An. triannulatus in Central Brazil

Abstract

Anopheles halophylus Silva-do-Nascimento & Lourenc o-de-Oliveira was recently described using morphological and biological variants in specimens previously identi ed as Anopheles triannulatus (Neiva & Pinto). Because these two species occur in sympatry in central Brazil, we used allozymes to determine the extent of gene flow to confirm that they are different species. Of 11 allozyme loci analyzed, one (Mpi) was found to be diagnostic for An. halophylus and An. triannulatus, confirming their speci c status. This locus revealed a second sibling species within An. triannulatus sensu lato. An. halophylus and the new undescribed species were con rmed using random ampli ed polymorphic DNA markers that showed moderate genetic divergence among these three sympatric and closely related taxa (D 0.145 0.428). Moreover, this marker indicates that An. halophylus and the new species are more closely related to each other than either is to An. triannulatus.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2006
Accession Number
ADA505409

Entities

People

  • F. A. Monteiro
  • R. C. Wilkerson
  • R. Lourenco-de-oliveira
  • T. F. Silva-do-nascimento

Organizations

  • Walter Reed Army Institute of Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Argentina
  • Brackish Water
  • Central America
  • Chain Reactions
  • Chemical Reactions
  • Entomology
  • Enzymes
  • Genes
  • Genetics
  • Genitalia
  • Islands
  • Personality
  • Polymerase Chain Reaction
  • South America
  • Trinidad
  • West Indies

Readers

  • Snow Cover Descriptors for Reptiles and Their Illustrations.
  • Vector-Borne Disease and Entomology

Technology Areas

  • Biotechnology