Early Systemic Biomarkers of Acute Lung Injury: Application of Multiplex Proteomic Array Technology
Abstract
The primary objectives of the present study were: 1) to set up a non-invasive animal model of 'inhalation injury', to monitor early changes in levels of pro-inflammatory cytokines in breath condensates of live rats, to delineate early biomarkers of pulmonary inflammation; 2) to further investigate the role of specific proinflammatory mediator(s) in acute lung inflammation/injury following exposure to toxic fire gases and/or BOP shockwave-mediated blunt trauma; 3) to monitor changes in various pro-inflammatory cytokines/chemokines in small volumes of biological samples (10-50 microliters), by utilizing a highly sensitive Proteome Array-based Multiplex ELISA; 4) to identify early systemic 'biomarkers' of acute lung injury/toxicity, for development of much-needed strategies/tools for effective therapeutic intervention and lung-health assessment of active-duty U.S. solders deployed around the world. The primary goal of this study was to identify early 'biomarkers' of acute pulmonary inflammation, as an predictive indicator of acute lung toxicity and/or injury, following exposure of rats to a brief high-dose of NO2, a militarily relevant toxic fire gas and a product of high temperature combustion.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2008
- Accession Number
- ADA505713
Entities
People
- Adolph Januszkiewicz
- Jayasree Nath
Organizations
- Walter Reed Army Institute of Research