Identification of Anopheles (Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes

Abstract

Anopheles (Nyssorhynchus) marajoara is a proven primary vector of malaria parasites in Northeast Brazil. and An. deaneorum is a suspected vector in Western Brazil. Both are members of the morphologically similar Albitarsis Complex. which also includes An. albitarsis and an undescribed species, An. albitarsis "B. These four species were recognized and can be identified using random amplified polymorphic DNA (RAPD) markers. but various other methodologies also point to multiple species under the name An. albitarsis. We describe here a technique for identification of these species employing polymerase chain reaction (PCR) primers based on ribosomal DNA internal transcribed spacer 2 (rDNA ITS2) sequence. Since this method is based on known sequence it is simpler than the sometimes problematical RAPD-PCR. Primers were tested on samples previously identified using RAPD markers with complete correlation.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2005
Accession Number
ADA506129

Entities

People

  • Cong Li
  • Richard Charles Wilkerson

Organizations

  • Walter Reed Army Institute of Research

Tags

DTIC Thesaurus Topics

  • Amplification
  • Argentina
  • Chain Reactions
  • Chemical Reactions
  • Dna Sequence Analysis
  • Genetics
  • Genitalia
  • Identification
  • Polymerase Chain Reaction
  • Population Genetics
  • Sequence Analysis
  • Sequences
  • South America
  • Thermal Cyclers
  • Venezuela

Fields of Study

  • Environmental science

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