Molecular Basis of Autophagic Cell Death in Prostate Cancer

Abstract

To understand the molecular basis of autophagy in prostate cancer, I am trying to isolate pure autophagosomes from autophagic cell survival and autophagic cell death Prostate cancer cell model and profile proteins and lipids to identify autophagy abundant/specific proteins and lipid molecule that are essential for functional autophagy in prostate cancer. I established EGFP.LC3 stably expressing LNCaP cell line and characterized the autophagic activity by western blot, translocation assay and Atg5 siRNA transfection. I also confirmed the autophagic activity in PC3.EGFP.LC3 cells. I was able to enrich autophagosomes using gradient ultracentrifugation and currently trying to optimize the condition for further purification by Immunopurification using beads.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2009
Accession Number
ADA506319

Entities

People

  • Ramesh Kaini

Organizations

  • University of New Mexico

Tags

DTIC Thesaurus Topics

  • Autophagy
  • Biomedical And Dental Materials
  • Biomedical Research
  • Cell Line
  • Cell Membrane
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Polymer Chemistry
  • Polymeric Films
  • Programmed Cell Death
  • Prostate Cancer
  • Proteins
  • Proteomics

Fields of Study

  • Biology
  • Chemistry

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics
  • Molecular and Cellular Biochemistry