O-GlcNAc Misregulation and Aneuploidy in Breast Cancer

Abstract

We examined the effects of siRNA depletion of Early Mitotic Inhibitor 1(Emi1) with O-GlcNAc transferase(OGT) and O-GlcNAcase(NCOAT) on re-replication and found a modest increase in rereplication with NCOAT/Emi1 knockdown. By western analysis our knockdown has been modest, and we have further optimization of the siRNA conditions to perform. We have identified a set of microRNAs whose expression are regulated by DNA damage in breast cancer, including miR-29c, which has been shown to play a role in regulating cell survival. We have found that miR-29c expression is induced in multiple cell lines following DNA damage, and its basal expression is affected by the p53 status of the cell line. We intend to examine whether miRNAs involved in cell survival following DNA damage can act synergistically with re-replication generated by Emi1 knockdown and interference with O-GlcNAc signaling leading to malignant transformation.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2009
Accession Number
ADA506322

Entities

People

  • Adam Mueller

Organizations

  • University of Virginia

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Chromosome Aberrations
  • Epithelial Cells
  • Genomic Instability
  • Inhibitors
  • Ionizing Radiation
  • Neoplasms
  • Radiation
  • Survival

Fields of Study

  • Biology

Readers

  • Allergy and Immunology.
  • Molecular Biology and Genetics