Detection of Tumor Suppressor Gene Mutations on 17p in DCIS
Abstract
The most powerful indicator of the location of TSGs in sporadic breast tumors has come from LOH studies. The implication is that a recessive mutation in the gene is "exposed" because the normal gene has been lost. DCIS is considered a precursor lesion of infiltrating ductal carcinoma (IDC). LOH at 17p is a recurrent observation specific to grade III DCIS and Grade III IDC suggesting a role for these alterations in tumor progression. Since loss of 17p is categorically related to both high grade DCIS and high grade IDC this region more than likely harbors one or more tumor suppressor genes involved in the progression of DCIS to IDC. High-density oligonucleotide arrays offer the ability to sequence large numbers of loci in parallel using an automated approach. There are many examples where array-based sequencing has proved successful, however, most of these applications have used normal samples for the identification of SNPs in specific chromosomal regions. The CustomSeq Arrays enable the analysis of 300kb stranded sequence on a single array. This provides the most cost effective and efficient scheme to query large amounts of sequence in a single experiment. . Our plan is to use this technology to search for mutations at 17p13 in IDC cells that display loss of this region, suggesting the presence of mutated TSGs.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2008
- Accession Number
- ADA506387
Entities
People
- Lesleyann Hawthorn
Organizations
- Health Research, Incorporated