Mechanisms of Aging of Phosphylated Serine Hydrolases

Abstract

The overall goal was to study mechanisms of aging of serine esterases inhibited by organophosphorus compounds capable of producing acute and delayed neurotoxicity. Acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and neuropathy target esterase catalytic domain (NEST) were reacted with diisopropylphosphorofluoridate (DFP) and N,N'-diisopropylphosphorodiamidofluoridate (mipafox, MlP). Bimolecular rate constants of inhibition and first-order rate constants of aging were determined colorimetrically. Phosphyl adducts of inhibited and aged.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 25, 2009
Accession Number
ADA509083

Entities

People

  • Rudy J. Richardson
  • Sanjeeva J. Wijeyesakere

Organizations

  • University of Michigan

Tags

Communities of Interest

  • Weapons Technologies

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Agreements
  • Biochemistry
  • Chemistry
  • Computational Modeling
  • Crystal Structure
  • Dynamics
  • Enzymes
  • Hydrolases
  • Inhibition
  • Kinetics
  • Mass Spectra
  • Mass Spectrometry
  • Membrane Lipids
  • Neuropathy
  • Organophosphorus Compounds
  • Spectrometry

Readers

  • Neurotoxicology