CYP1B1 Polymorphism as a Risk Factor for Race-Related Prostate Cancer. Addendum

Abstract

In the first hypothesis, CYP1B1 expression in Caucasian prostate cell lines were increased in cancerous (DU145 & PC-3) compared to normal (RWPE-1) cells. Analysis of expression in human tissue cDNAs from Caucasians also showed higher levels of CYP1B1 in cancer compared to BPH. CYP1B1 protein was present in both races and though not significant, generally was higher in tumor regions compared to normal adjacent regions for both African-Americans and Whites. In the second hypothesis, racial differences for CYP1B1 polymorphisms are observed as allele frequencies for the variant at codons 119 and 432 are greater among Blacks (P<0.001) whereas the 453 variant is predominant in Whites (P<0.001). Within race, a case control study show the variant at codon 453 plays a protective role for PC among Blacks (P<0.05). Interestingly, SNPs at codons 432 and 449 are determined to be linked and the 432G-449C haplotype was observed to be a risk for PC (P<0.05). In a sampling of cases, no differences were observed between stages (<T2c vs >T2c) and grades (<7 vs >7) of PC in either race. In the remaining year, more SNP studies with additional samples to be collected; as well as further experimentation with aim #1 will be performed.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2009
Accession Number
ADA510133

Entities

People

  • Rajvir Dahiya
  • Yuichiro Tanaka

Organizations

  • Northern California Institute for Research and Education

Tags

DTIC Thesaurus Topics

  • African Americans
  • Biomedical Research
  • Caucasians
  • Cell Line
  • Cells
  • Chemical Reactions
  • Chi Square Test
  • Frequency
  • Gel Electrophoresis
  • Gene Expression
  • Medical Personnel
  • Neoplasms
  • Polymerase Chain Reaction
  • Prostate
  • Prostate Cancer
  • Risk Factors
  • Tissues

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