Regulatory T Cells and Host Anti-CML Responses

Abstract

CD4+CD25+FoxP-3+ regulatory T-cells (Tregs) suppress immune responses to 'self' antigens, but also have been shown to suppress host anti-tumor responses in several human malignancies, including breast, gastrointestinal, and ovarian cancer. Identification of CML Tregs as suppressors of host anti-CML responses could have a significant impact upon CML treatment strategies. Methods are currently available to selectively suppress Tregs and subsequently boost host anti-CML responses. We have examined the CD4+CD25+FoxP-3+ regulatory T-cell population in the peripheral blood from healthy individuals and those with CML using flow cytometry. Our results demonstrate that subjects with CML who have detectable residual disease have a larger percentage of CD4's that are Tregs (6.89% +/- 3.32 vs 1.94% +/- 0.99, p 0.003)and a >3.5 fold larger absolute number of circulating CD4+CD25+FoxP3+ T-cells (1,743 +/- 1,350 vs 455 +/- 234, p0.02), consistent with our hypothesis. The larger numbers of circulating Treg's appears to correlate with CML disease activity. We are continuing to examine functional correlates of this CML Treg population.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2009
Accession Number
ADA510759

Entities

People

  • K. K. Wong Jr.

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Blood
  • Cancer
  • Cells
  • Diseases And Disorders
  • Identification
  • Institutional Review Board
  • Lymphocytes
  • Medical Personnel
  • Neoplasms
  • Ovarian Cancer
  • Residuals
  • Stem Cells
  • Suppressors
  • T Lymphocytes

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology