Obstructing Androgen Receptor Activation in Prostate Cancer Cells through Posttranslational Modification by NEDD8
Abstract
In this study, we aim to investigate post-translational modification of the androgen receptor (AR) by a small ubiquitin-like protein, Nedd8. We planned to analyze the consequence of Nedd8 modification on AR activity, and to identify the neddylation sites on AR. We also planned to determine enzymes involved in AR neddylation and de-neddylation. We have completed all proposed tasks in the first year. Key accomplishments so far include the development of a protocol to enrich neddylated form of AR, the demonstration that a portion of endogenous AR is modified by Nedd8, and the finding that androgen reduces AR neddylation. Reportable outcomes include the preparation of a manuscript and the award of a PhD degree to a graduate student. We expect to continue the research into the second year and no major changes on future work are necessary.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2009
- Accession Number
- ADA510799
Entities
People
- J Chen D.
Organizations
- Robert Wood Johnson Medical School