Biological Sensors Using DNA Functionalized Multiwalled Carbon Nanotubes
Abstract
In the first part of this work, electrochemical detecting behavior of MWNT functionalized with single strand DNA was studied. The MWNT was synthesized as described in the report. Purified MWNT was functionalized with aqua regia for dispersion improve combining capacity with the DNA. Special machine grade DNA [AC] 15 was used for functionalization of MWNT by sonication. Characterizations have been carried out using various techniques. Cyclic voltammetry gave well-defined quasi-reversible voltametric responses, showing that DNA-MWNT film is a good electrochemical sensor. Further, we demonstrate highly selective and sensitive detection of a low concentration of dopamine. In the second part of this work, a new amperometric biosensor, based on deposition of glucose oxidase (GOD) on to crystalline gold (Au) nanoparticle modified multi walled carbon nanotube (MWNT) electrode, is presented. Purified MWNTs were decorated with nanocrystalline Au metal clusters using a simple chemical reduction method. Amperometric biosensor fabricated by depositing glucose oxidase (GOD) over Nafion-solubilized Au/MWNT electrode retains its biocatalytic activity and offers fast and sensitive glucose quantification.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2009
- Accession Number
- ADA511260
Entities
People
- A. Leela Mohana Reddy
- S. Ramaprabhu
- Samiran Ganguly
Organizations
- Indian Institute of Technology Madras