The Role of BRCA1 Domains and Motifs in Tumor Suppression

Abstract

The purpose of this research is to classify BRCA1 variants for which cancer association is not known (unclassified variants UCV). To approach this problem we hypothesized that poorly characterized but conserved domains in BRCA1 directly participate in its tumor suppression function. To test this hypothesis we choose a global approach analyzing several BRCA1 domains and point mutants in functions that have previously been attributed to BRCA1: long term survival after irradiation, early G2/M checkpoint, intra S phase checkpoint, and spindle assembly checkpoint. We successfully optimized conditions for expression of full-length BRCA1 mutants in two different cell lines by electroporation. We also optimized all the checkpoints assays in our laboratory and are now ready to analyze BRCA1 mutants proposed in this grant. This will have a significant impact not only to understand BRCA1 role as a tumor suppressor in breast cancer but also to help patients that are carriers of BRCA1 mutation to make informed clinical decisions.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2009
Accession Number
ADA511277

Entities

People

  • Aneliya Velkova

Organizations

  • H. Lee Moffitt Cancer Center & Research Institute

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antineoplastic Agents
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Cytoskeleton
  • Fungi
  • Genetics
  • Neoplasms
  • Organic Chemistry
  • Proteins
  • Tumor Cell Line

Readers

  • Breast cancer cell signaling and growth regulation.
  • Immunology
  • Parallel and Distributed Computing.