Isolation of Polymorphic Microsatellite Markers from the Malaria Vector Anopheles marajoara (Diptera: Culicidae)

Abstract

Microsatellite-containing regions were isolated and characterized in Anopheles (Nyssorhynchus) marajoara, a primary vector of malaria parasites in northeastern Amazonia, Brazil. An enrichment protocol yielded 500 positive clones. We designed primers to amplify 40 unique microsatellites, 11 of which amplified cleanly and were polymorphic. A survey of 323 individuals showed that these loci are highly variable with the number of alleles ranging from 11 to 52, and expected heterozygosity ranging from 0.64 to 0.95. These markers will be useful for studies of population structure and intraspecific variation in A. marajoara.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2005
Accession Number
ADA511487

Entities

People

  • Cong Li
  • Dina M. Fonseca
  • Richard Charles Wilkerson

Organizations

  • Walter Reed Army Institute of Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Artificial Satellites
  • Biology
  • Chain Reactions
  • Chemical Reactions
  • Chromosomes
  • Costa Rica
  • Data Analysis
  • Department Of Defense
  • Electronic Mail
  • Genetic Structures
  • Genetics
  • Microsatellites
  • Molecular Biology
  • New England
  • North America
  • Polymerase Chain Reaction
  • Population Genetics

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