Identification of Chromosome 18q Transcripts Lost in Breast Cancer
Abstract
A novel recurrent homozygous region of loss at 18q22.3 was detected in 50% of breast tumors by array comparative genomic hybridization. Since chromosomal regions exhibiting homozygous deletion are rare and are usually the site of tumor suppressor genes, the following hypothesis was proposed: Encoded within the region of homozygous deletion at 18q22.3 is a transcript whose loss plays a role in the development or progression of breast cancer. A custom oligonucleotide expression microarray covering the deleted region was designed and synthesized. RNA was isolated from primary human mammary epithelial cells (HMEC; Lonza), converted to fluorescently-labeled complementary RNA and hybridized to the microarray. An approximately 500 basepair sequence was found to be transcribed in breast epithelial cells. This is a novel transcript that has not been described previously. We confirmed this transcript with a quantitative PCR assay using normal breast epithelial cell cDNA. We also detected this transcript in brain, cervix, spleen and thymus tissues. To identify the full-length transcript, a brain cDNA library was screened.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2009
- Accession Number
- ADA511855
Entities
People
- Teresa L Johnson-Pais
Organizations
- University of Texas Health Science Center at San Antonio