Molecular Genetic and Gene Therapy Studies of the Musculoskeletal System

Abstract

The proposed research projects focuses on bone health, including relevance to the musculoskeletal system in battlefield performance and in battlefield injury. We have utilized state-of-the-art molecular genetic and gene therapy technologies to address fundamental questions in bone biology with particularly emphasis on attempting to clarify gene function for those genes that are involved in digit tip regeneration, bone size, skeletal repair and anabolic response to mechanical loading. The molecular genetic approaches have utilized various knockout mouse models and whole genome microarray analysis for evaluation of gene functions. The gene therapy approaches have utilized various viral and non-viral vectors to transfer gene of interest to treat localized and systemic skeletal deficits. We have successfully accomplished nearly all of the objectives proposed in the grant. The four core facilities funded by this project have contributed enormously to the success of our research activities. Our research activities during the past funding period have lead to 14 publications and 14 abstracts. Our studies have led to the identification of key genes and their pathways that are involved in skeletal regeneration and repair. Furthermore, we have developed successful gene therapy application to promote healing of skeletal injuries and increase bone formation throughout the skeleton.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2009
Accession Number
ADA512941

Entities

People

  • Subburaman Mohan

Tags

DTIC Thesaurus Topics

  • Bone And Bones
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Genetics
  • Health Services
  • Medical Personnel
  • Peptide Growth Factors

Readers

  • Immunology and Pathology
  • Molecular Genetics
  • Technical Research and Report Writing.

Technology Areas

  • Biotechnology