Prolactin and Human Ovarian Cancer
Abstract
Multiple prolactin receptors (PRLRs) derived from differential splicing of the same gene transcript transduce very different PRL-initiated effects on cells. In this project, we determined the kinds of PRLRs expressed by three human ovarian cancer cell lines: TOV-112D, OV-90 and TOV-21G. Using quantitative PCR and Western blot, we established that the long form (LF) and both short forms (SF1a and SF1b) of the human PRLRs were expressed by all three cell lines. To clarify the functional role of these receptors, we used three different ligands for the receptor, one which in other systems promotes proliferative (unmodified PRL, UPRL), one which acts as a competitive antagonist (G129R), and one, a selective activator (S179D). When cells were cultured in medium containing 10% FBS, UPRL had no effect on viable cell number while both of the other ligands reduced cell number. When FBS was omitted from medium, UPRL increased, while G129R and S179D decreased cell number. Further analysis showed UPRL decreased apoptosis and promoted cell migration, while S179D increased apoptosis and inhibited cell migration. The survival response and increased migration with UPRL and decrease in cell number and decreased migration in response to S179D were exaggerated by over expression of LF and SF1b.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2009
- Accession Number
- ADA513058
Entities
People
- Dunyong Tan
Organizations
- University of California, Riverside