Whole-Genome Single Nucleotide Polymorphism Based Phylogeny of Francisella tularensis and Its Application to the Development of a Strain Typing Assay

Abstract

A low genetic diversity in Francisella tularensis has been documented.. Current DNA based genotyping methods for typing F. tularensis offer a limited and varying degree of subspecies, clade and strain level discrimination power. Whole genome sequencing is the most accurate and reliable method to identify, type and determine phylogenetic relationships among strains of a species. However, lower cost typing schemes are necessary in order to enable typing of hundreds or even thousands of isolates.

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Document Details

Document Type
Technical Report
Publication Date
Oct 07, 2009
Accession Number
ADA513240

Entities

People

  • Claudia Molins
  • Gagan A. Pandya
  • Jeannine M. Petersen
  • Marcus Jones
  • Mark J. Wolcott
  • Martin E. Schriefer
  • Michael H. Holmes
  • Robert D. Fleischmann
  • Scott N. Peterson
  • Sonal Pradhan
  • Svetlana A. Karamycheva

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Biology
  • Biomedical Research
  • California
  • Data Analysis
  • Deoxyribonucleic Acids
  • Discrimination
  • Gammaproteobacteria
  • Genetic Variation
  • Genetics
  • High Resolution
  • Infectious Diseases
  • Microbiology
  • New York
  • North America
  • Nucleotides
  • Polymerase Chain Reaction
  • United States

Fields of Study

  • Biology

Readers

  • Infectious Disease/Epidemiology
  • Molecular Genetics
  • Regression Analysis.

Technology Areas

  • Biotechnology