Dissecting the Molecular Mechanism of RhoC GTPase Expression in the Normal and Malignant Breast
Abstract
Primary inflammatory breast cancer (IBC) accounts for approximately 3% of new breast cancers in the US. This form of locally advanced breast cancer is characterized clinically by erythema, warmth, and dimpling of the skin that arise rapidly. In fact, IBC is not associated with precursor lesions, but is rapidly invasive, highly angiogenic and metastatic from onset. IBC has previously been characterized by the Merajver lab to have increased RhoC expression as compared to stage matched controls. Despite this the molecular mechanisms giving rise to the disease, and to RhoC overexpression, have not been characterized. In this project, we have postulated several potential molecular mechanisms that may lead to RhoC overexpression. Thus far, we have developed a method for gene fusion identification from paired end sequencing data, and used SOLEXA high throughput massively parallel sequencing to identify potential gene fusion candidates in two IBC cell lines. Likewise, we have also completed aCGH and microRNA profiling analysis of these cell lines. Unexpectedly, we found that two IBC cell lines both harbor an extra copy of Chromosome1 and are currently studying the significance of this association.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2009
- Accession Number
- ADA513338
Entities
People
- John Brenner
Organizations
- University of Michigan