Enzyme-Mediated Assimilation of DNA-Functionalized Single-Walled Carbon Nanotubes

Abstract

When pyrimidine-functionalized carbon nanotubes were incubated with single-stranded DNA ligase, formations of macroscopic aggregates were observed. Wet-cell transmission electron microscopy imaging revealed that the nanotubes were radially bound to form a 3D latticelike structure. These structures were not observed in control reactions lacking ligase or adenosine triphosphate. Raman spectroscopy analysis revealed no spectra indicative of carbon nanotubes in ligase-unamended controls; however, spectra were observed in radial breathing mode and in the G and G' bands in reactions containing ligase. Furthermore, the addition of deoxyribonuclease to the ligated reactions dispersed the aggregates, and a reduction in Raman spectral intensity was observed.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2010
Accession Number
ADA513373

Entities

People

  • Charles P. Marsh
  • Charles R. Welch
  • Clint M. Arnett
  • Jae-hee Han
  • Jeffry H. Gray
  • Michael Strano
  • Thomas A. Carlson

Organizations

  • Engineer Research and Development Center

Tags

Communities of Interest

  • Advanced Electronics

DTIC Thesaurus Topics

  • Assimilation
  • Carbon Nanotubes
  • Chemistry
  • Electron Microscopy
  • Engineering
  • Fullerenes
  • Materials
  • Microscopes
  • Microscopy
  • Nanomaterials
  • Nanotechnology
  • Raman Spectra
  • Raman Spectroscopy
  • Spectra
  • Spectroscopy
  • Transmission Electron Microscopy
  • Wet Cells

Fields of Study

  • Physics

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Nanocomposite Materials Science
  • Solar Physics

Technology Areas

  • Microelectronics