Ricin Activity Assay by Direct Analysis in Real Time Mass Spectrometry Detection of Adenine Release
Abstract
Biotoxin activity assays typically involve multistep sample preparation, multicomponent reactions, multistep analysis, or a combination thereof. We report a single-step, real time ricin activity assay that requires little or no sample preparation and employs direct analysis in real time mass spectrometry. The release of adenine from the inhomogeneous substrate herring sperm DNA by ricin was determined to be 53 +/- 2 pmol adenine per picomole of ricin per hour. This procedure can be readily adapted to any enzyme for which a reactant or product of low molecular weight (up to ~600) can be identified.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2010
- Accession Number
- ADA513378
Entities
People
- Amanda M. Schenning
- H. Dupont Durst
- J. M. Nilles
- Jeffrey S. Rice
- Lisa M. Reilly
- Theresa R. Connell
- Vicky L.H. Bevilacqua
Organizations
- Edgewood Chemical Biological Center