Metabolic Reorganization in Breast Cancer Epithelial Cells: Role of the Pentose Phosphate Shunt

Abstract

Breast cancer is the most frequently diagnosed cancer in women with about 180,000 new cases reported each year. Early detection allows the greatest chance for successful therapies which include surgical procedures, irradiation, hormonal, and chemical intervention. Yet, these do not always achieve complete recovery, so our goal was to develop novel techniques that might identify markers that would allow us to construct metabolic maps in different types of breast cancers to predict efficacy of therapeutic treatment options. Using NMR, we have now demonstrated that hypoxic treatment of a basal B, triple negative breast cancer cell line increases substantially the flux of non-glycolytic products into the TCA cycle which might increase the ability of cells to use oxidative phosphorylation for ATP production. If we can identify these products, we may be able to block their entry into TCA cycle to compromise the cells ability to supplement their energy and block their selective advantage.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2009
Accession Number
ADA514032

Entities

People

  • Art Edison
  • Nick Simpson
  • Susan C. Frost

Organizations

  • University of Florida

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Detection
  • Diseases And Disorders
  • Epithelial Cells
  • Glutamates
  • Lactic Acid
  • Metabolism
  • Neoplasms
  • Phosphorylation
  • Production
  • Surgery
  • Therapy

Readers

  • Aquatic Ecology
  • Oncology (Cancer Research).