Toxicity of Trinitrotoluene to Sheepshead Minnows in Water Exposures
Abstract
Lethal effects of trinitrotoluene (TNT) to juvenile sheepshead minnows (JSHM) (Cyprinodon variegatus) were assessed in ten-day water exposures. Ten-day median lethal concentrations (LC50s) were 2.3 and 2.5 mg/L, the 10-d median lethal residue value (LR50) was 26.1 micromol/kg wet weight (ww), and bioconcentration factors (BCFs) ranged from 0.7 to 2.4 L/kg. The lethal effects of TNT and its transformation products 2-aminodinitrotoluene (2-ADNT), 2,4-diaminonitrotoluene (2,4-DANT) and trinitrobenzene (TNB) to JSHM were compared in 5-d static-renewal exposures. Nitroreduction decreased the toxicity of TNT to SHM, as the 5-d LC50 for 2-ADNT was 8.6 mg/L and the lowest lethal concentration of 2,4-DANT was 50.3 mg/L. TNB (5-d LC50=1.2 mg/L) was more toxic than TNT to SHM. The 5-d LR50s were 4.3 mg/kg ww (20.4 micromol/kg) for SumTNT (TNT exposure) and 54.2 mg/kg ww (275.3 mmol/kg) for 2-ADNT and significant mortality occurred at 47.4 mg/kg ww (283.6 micromol/kg). The range of BCF values was from 1.8 to 2.4, 5.6 to 8.0, and 0.6 to 0.9 L/kg for TNT, 2-ADNT, and 2,4-DANT, respectively.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 06, 2010
- Accession Number
- ADA515578
Entities
People
- Alfreda B. Gibson
- Guilherme R Lotufo
- William M. Blackburn
Organizations
- Engineer Research and Development Center