Regulation of the Inflammasome, a Modulator of Caspase-1 Mediated Cytokine Production

Abstract

Tissue inflammation and inflammatory cytokines can positively affect breast cancer prognosis. By providing a detailed understanding of the mechanism of inflammasome formation and activation, we hope to create the potential for inflammation based cancer therapies. For each of the key protein domains in the inflammasome complex, panels of constructs have been screened for their amenity to further biochemical characterization. NALP LRR domain was found to express poorly, with poor solubility, possibly due to oxidation-related folding problems. Constructs of the adaptor CARDINAL were found to express well, but suffer proteolysis during purification. Analysis of CARDINAL partial proteolysis products may yield a proteolyticaly stable CARDINAL subdomain. NACHT constructs were successfully produced by baculovirus/SF9 expression, and will be subjected to biochemical and enzymatic characterization.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2007
Accession Number
ADA517127

Entities

People

  • Karl Schmitz

Organizations

  • University of Pennsylvania

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Baculoviridae
  • Biomedical Research
  • Breast Cancer
  • Cells
  • Chemical Reactions
  • Cysteine
  • Cytokines
  • Diseases And Disorders
  • Escherichia Coli
  • Inflammasomes
  • Inflammation
  • Mass Spectroscopy
  • Neoplasms
  • Oxidation
  • Proteins
  • Solubility

Readers

  • Approximation Theory.
  • Molecular Biology and Genetics
  • Trauma Surgery or Emergency Medicine.