Development of the Electrochemical Biosensor for Organophosphate Chemicals Using CNT/Ionic Liquid Bucky Gel Electrode

Abstract

Organophosphorus hydrolase (OPH) immobilized on CNT/ionic liquid (IL) electrodes were prepared by using three different intrinsic kinds of ILs, as binders. CNTs/ILs lead to dramatic electrochemical enhancements with respect to response time, stability, and sensitivity of composite electrodes. In addition, the electrochemical and biocatalytic properties of three-composite electrodes were strongly influenced by different types of ILs used, as verified by cyclic voltammetry and chronoamperometry. These results were attributed to the conformational changes of the microenvironment between the OPH and the composite electrodes within three different types of ILs. In particular, the biocatalytic signals of three OPH/CNT/ILs modified electrodes increased linearly to the concentration of paraoxon in a wide range of 2?20 lM. These findings provide a deep understanding of the role of each IL on the modified electrodes, enabling to enhance electrochemical properties for biosensors.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2010
Accession Number
ADA517373

Entities

People

  • Bong G. Choi
  • Dong Hun Kim
  • Hoseok Park
  • Sang Y Lee
  • Tae Joon Park
  • Won H. Hong

Organizations

  • KAIST

Tags

Communities of Interest

  • Biomedical
  • Weapons Technologies

DTIC Thesaurus Topics

  • Biological Sciences
  • Biosensors
  • Carbon Nanotubes
  • Chemical Warfare Agents
  • Composite Materials
  • Detection
  • Detectors
  • Electrochemical Reactions
  • Electrochemistry
  • Electrodes
  • Electron Transfer
  • Engineering
  • Fullerenes
  • Ionic Liquids
  • Materials
  • Measurement
  • Sensitivity

Fields of Study

  • Chemistry

Readers

  • Electrochemical Surface Science
  • Nanocomposite Materials Science
  • Neurotoxicology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech