Production and Characterization of a Novel OX40 Ligand for Clinical Use

Abstract

The goals of the first year/phase of the DOD funded research, PC073499, have been accomplished. We produced two human versions of the OX40 ligand:trimer:Ig protein. The two protein constructs have different human trimer domains (TRAF2 and Matrillin-4), which confer differing folding patterns within the two-dimension structure of the protein. Both the TRAF2 and Matrilin-4 trimer domain constructs appear to have as good if not better in vitro bioactivity when compared to a mouse anti-human OX40 Ab that is currently in a clinical trial for cancer patient treatment. We have produced enough purified material to test the in vivo activity of these proteins and have planned a pilot study involving injection of these new human OX40 agonists in non-human primates. Upon completion of this project we will have important preclinical information that should allow for FDA approval of a fully human OX40 agonist to be tested in a clinical trial.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2009
Accession Number
ADA517909

Entities

People

  • Andrew D. Weinberg

Tags

DTIC Thesaurus Topics

  • Assays
  • Biomedical Research
  • Cell Physiological Processes
  • Cells
  • Clinical Trials
  • Column Chromatography
  • Department Of Defense
  • Gel Electrophoresis
  • High Resolution
  • Lymphocytes
  • Materials
  • Neoplasms
  • Pilot Studies
  • Production
  • Prostate Cancer
  • Proteins
  • Recombinant Proteins

Fields of Study

  • Biology

Readers

  • Analytical Mechanics
  • Clinical Trial Research.
  • Molecular Genetics