In Vivo Test for Chemical Induction of Micronucleated Polychromatic Erythrocytes in Mouse Bone Marrow Cells, Test Article: Ethylenediamine Dinitrate (EDDN)
Abstract
Ethylenediamine dinitrate (EDDN) (99.5% pure) was tested forclastogenic potentials to induce micronucleated polychromatic erythrocytes (MPCE) in the bone marrow cells of male and femal CD-1 mice according to OECD TG 474 in compliance with Good Laboratory Practice. Male and female mice were dosed orally at 500, 1000, and 2000 mg/kg. Animals were euthanized approximately 24 and 48 hr after dosing. The percentage of polychromatic erythrocytes (PCE) and frequency of micronucleated polychromatic erythrocyte (MPCE) were determined at 24 and 48 hours. Cytotoxicity was assessed by scoring the number of PCE and norchromatic erythrocyte (NCE) in first 200 erythrocytes for each animal. There were not statistically significant increases in the number of MPCE in the treated groups when compared to the concurrent vehicle control group. These results indicate that EDDN was negative in the in vivo mouse micronucleus assay and therefore is not considered to be a clastogenic agent.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 05, 2009
- Accession Number
- ADA518233
Entities
People
- Karen Shore
- Paul Kirby