Test for Chemical Induction of Chromosome Aberrations in Cultured Chinese Hamster (CHO) Cells With and Without Metabolic Activation, Test Acticle: Ethylenediamine Dinitrate (EDDN)
Abstract
Ethylenediamine dinitrate (EDDN) (99.5% pure) was tested for clastogenic potential to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice,. EDDN was tested at concentrations 500, 1000, 2000, 3000, 4000 and 5000 ug/mL both with and without activation. Based on the cytotoxicity data, chromosome aberrations were scored from the cells treated with the concentrations 1000, 2000, and 3000 ug/mL without activation and 3000, 4000, and 5000 ug/mL with activation. Two hundred metaphases were scored from each concentration and controls. EDDN did not induce a statistically significant increase in the percentage of chromosome aberration, polyploidy and endoreduplicated cells in the CHO cells both with and without activation. A confirmatory chromosome aberration assay was performed without activation also showed negative.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 25, 2010
- Accession Number
- ADA518238
Entities
People
- Jian Song
- Paul E. Kirby