Self-Reporting and Detoxifying Materials Based on Extremophilic Proteins
Abstract
The central aim of this project was to utilize "extremophilic" proteins in the fabrication of robust biomaterials. Specific objectives included the development of enabling components that will impart biomaterials with the ability to sense failure and/or repair defects, and the construction of new protein shapes by programmed self-assembly. The biomolecular constituents of these systems are highly thermostable and solvent-resistant chaperone proteins capable of refolding and reactivating denatured proteins under extreme conditions, and a unique filamentous protein recently discovered in the deep-sea hyperthermophile Methanocaldococcus jannaschii (the gamma-PFD). The project goals were successfully met through several specific accomplishments, including development of a FRET-based nanosensor for damage-reporting polymeric materials, creation of a partially self-renaturing enzymatic fusion protein, preparation of protein-templated nanowires from the thermostable gamma-PFD, and engineering of the gamma-PFD scaffold to form nanoscale ovaloids, a new protein architecture.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2010
- Accession Number
- ADA518828
Entities
People
- Douglas S Clark
Organizations
- University of California, Berkeley