Evaluation of a Test Article in the Salmonella typhimurium/Escherichia coli Plate Incorporation Mutation Assay in the Presence and Absence of Induced Rat Liver S-9. Test Article 3-Nitro-1,2,4-Triazol-5-one (NTO)
Abstract
3-Nitro-1,2,4-triazol-5-one (NTO, 99.6% pure) was tested for mutagenic potentials with Salmonella typhimurium strains, TA 98, TA 100, TA 1535, TA 1537 and Escherichia coli strain WP2 uvrA by plate incorporation method according to OECD TG 471 in compliance with Good Laboratory Practice. NTO was tested at concentrations of 5, 10, 50, 100 and 250 g/plate for Salmonella strains and 100, 250, 500, 750 and 1000 g/plate for Escherichia coli without activation. The dose levels were 100, 500, 1000, 2500 and 5000 g/plate for both Salmonella and Escherichia coli with activation. The results showed that NTO was not mutagenic in Salmonella typhimurium and Escherichia coli both with and without activation. The negative result was also verified by a confirmatory mutation assay both with and without activation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 17, 2008
- Accession Number
- ADA518833
Entities
People
- Jian Song