Analysis of Dachsous2 in Breast Cancer Progression and Recurrence
Abstract
Microarray analysis of tumors women with axillary node negative (ANN) breast cancer revealed that a cDNA for Dachsous 2 (Ds2) is one of the most highly discriminatory genes in tumors from patients who had experienced early recurrence, compared to tumors from patients who did not recur for at least ten years (p= 1.1 x 10-5 by students t-test). This suggests that increased transcription of Ds2 may be a predictive indicator of recurrence in ANN breast cancer. Ds2 encodes a large cell adhesion molecule1. The Drosophila homolog of Ds2, Ds, functions as a planar cell polarity (PCP) ligand for the large cadherin Fat(2-5), and together Ds and Fat regulate tissue organization through a PCP signaling pathway. Recent data has also linked Ds with a newly described growth control pathway, the Hippo kinase pathway(6-9). To determine the significance of the increased levels of Ds2 in recurrence we will: 1) Validate gene expression array results and determine if Ds2 proteins levels increase in tumors 2) Determine if PCP or Hippo pathway gene expression is altered upon Ds2 overexpression. 3) Determine the effects of altering Ds2 levels on proliferation and tumor susceptibility in the mouse.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2009
- Accession Number
- ADA519188
Entities
People
- Helen Mcneill
Organizations
- Mount Sinai Hospital, Toronto