In Vivo Test for Chemical Induction of Micronucleated Polychromatic Erythrocytes in Mouse Bone Marrow Cells. Test Article: N,N,N',N'-tetramethyl Ethanediamine (TMEDA)
Abstract
N,N,N(prime),N(prime)-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for its potentials to induce micronucleated polychromatic erythrocytes (MPCE) in the bone marrow cells of male and female CD-1 mice according to OECD TG 474 in compliance with Good Laboratory Practice. Male and female mice were dosed orally at 62.5,125 and 250 mg/kg. Animals were euthanized approximately 24 and 48 hrs after dosing. The percent of polychromatic erythrocytes (PCE) and frequency of micronucleated polychromatic erythrocyte (MPCE) were determined at 24 and 48 hrs. Cytotoxicity was assessed by scoring the number of PCE and normochromatic erythrocyte (NCE) in first 200 erythrocytes for each animal. There were no statistically significant increases in the number of MPCE in the treated groups when compared to the concurrent vehicle control group. These results indicate that TMEDA was negative in the in vivo mouse micronucleus assay and the compound was not clastogenic.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 19, 2008
- Accession Number
- ADA519471
Entities
People
- Jian Song
- Karen S. Shore