Evaluation of a Test Article in the Salmonella typhimurium/Escherichia coli Plate Incorporation Mutation Assay in the Presence and Absence of Induced Rat Liver S-9. Test Article: N,N,N',N'-tetramethyl ethanediamine (TMEDA)

Abstract

N,N,N',N'-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for mutagenic potentials with Salmonella typhimurium strains, TA 98, TA 100, TA 1535, TA 1537 and Escherichia coli strain WP2 uvrA by plate incorporation method according to OECD TG 471 in compliance with Good Laboratory Practice. TMEDA was tested at concentrations of 500, 750, 1,000, 3,000 and 5,000 g/plate for salmonella strains and Escherichia coli without and with activation. The results showed that TMEDA was not mutagenic in these strains both with and without activation. The result of confirmatory mutation assay with and without activation also showed negative response. TMEDA was negative in the Salmonella typhimurium/Escherichia coli plate incorporation mutation assay both with and without activation.

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Document Details

Document Type
Technical Report
Publication Date
Jun 12, 2008
Accession Number
ADA519620

Entities

People

  • Paul E. Kirby

Tags

DTIC Thesaurus Topics

  • Acceptability
  • Acquisition
  • Amino Acids
  • Bacteria
  • Chemistry
  • Computer Programs
  • Environment
  • Environmental Protection
  • Escherichia
  • Escherichia Coli
  • Indicator Dyes
  • Mutagenicity Tests
  • Mutations
  • Range Finding
  • Spreadsheet Software
  • Test And Evaluation
  • United States

Fields of Study

  • Biology

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  • Microbial Pathology
  • Toxicology/Environmental Toxicology