Identification of Pathogenic Vibrio Species by Multilocus PCR-Electrospray Ionization Mass Spectrometry and Its Application to Aquatic Environments of the Former Soviet Republic of Georgia
Abstract
The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA). All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2010
- Accession Number
- ADA520117
Entities
People
- Anwar Huq
- Carson Baldwin
- Christ A. Whitehouse
- Christopher Grim
- Ekaterina Jaiani
- Feng Li
- Heather Matthews
- Lawrence B. Blyn
- Marina Tediashvili
- Nino Janelidze
- Rachael Melton
- Rangarajan Sampath
- Rita R. Colwell
- Tamar Kokashvili
- Thomas A. Hall
- Vanessa Harpin
Organizations
- United States Army Medical Research Institute of Infectious Diseases