Mitotic Spindle Positioning in Breast Cancer

Abstract

The purpose of this project was to determine whether mitotic spindle position differs in benign versus malignant breast tissues, and to test whether estradiol alters spindle position. The most significant findings are that mammary tissue shows planar spindle orientation. Information about spindle position in breast cancer tissues for comparison is still pending, awaiting an additional collaboration. The other significant finding is that spindle orientation appeared random in MCF10-A cells and an endometrial cancer cell line (Ishikawa), but regulated in three dimensional cysts of MDCK cells. This regulation is sensitive to disruption of actin and microtubules, and to EGTA treatment. Now that the postdoc working on the project has left, I want to re-visit the experiments with MCF-10A cells using serum free media.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2009
Accession Number
ADA520543

Entities

People

  • Jennifer S. Tirnauer

Organizations

  • University of Connecticut Health Center

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Cultured Cells
  • Cytoskeleton
  • Epithelial Cells
  • Intercellular Junctions
  • Mammary Glands
  • Neoplasms
  • Orientation (Direction)
  • Polarity
  • Regulations
  • Three Dimensional
  • Uterine Cancers

Readers

  • Clinical Trial Research.
  • Molecular Biology and Genetics
  • Women's Health and Cancer Risk Research: African American Women and Pregnancy Outcomes.