Characterize RAP80, a Potential Tumor Suppressor Gene
Abstract
To examine the molecular mechanism by which BRCA1 participates in breast tumor suppression, we have identified that RAP80 is a BRCA1-associated protein by protein affinity purification. Here, we show the evidence that RAP80 controls BRCA1's relocation to DNA damage sites and regulates BRCA1-dependent DNA damage checkpoint function. Moreover, we have generated RAP80-deficient mice and analyzed the tumor phenotypes of the mice. In addition, we have screened RAP80 gene mutations in breast cancer cell lines. Our results indicate that RAP80 is a functional partner of BRCA1 in response to DNA damage. RAP80 is an important regulator to maintain genomic stability. However, mutations of RAP80 have not been identified to be associated with breast cancers. Our results have been published in Science (Vol. 316, 1202-1205) and Nature Structural and Molecular Biology (Vol. 14, 716-720).
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2009
- Accession Number
- ADA520785
Entities
People
- Xiaochun Yu
Organizations
- University of Michigan