A Polyamine Oxidizing Enzyme as a Drug to Treat Breast Cancer

Abstract

The purpose of the research is to test modified forms of bovine serum amine oxidase (SAO) as effective treatments for breast cancer, using a mouse model. If successful, this approach, or a variation thereof, may eventually be used as a therapy for breast and other cancers in humans. Currently, a large quantity of very pure bovine PAO is in hand, which was obtained from 10 gallons of fresh cow blood. A method has been developed for generating extremely pure enzyme, which will be deglycosylated before being polyethylene glycolated (PEGylated). PEGylated SAO will be tested for toxicity and for its ability to slow the growth or shrink the size of breast tumors implanted in test mice. PEGylated SAO should target tumors but have little effect on normal tissue. Once concentrated in a tumor, the active PEGylated SAO will oxidize acetylated polyamines, which are excreted by tumor cells in large quantities. When the acetylated polyamines are oxidized, cytotoxins are generated.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2006
Accession Number
ADA521388

Entities

People

  • William S. Mcintire

Organizations

  • Northern California Institute for Research and Education

Tags

DTIC Thesaurus Topics

  • Amines
  • Antineoplastic Agents
  • Biomedical Research
  • Blood
  • Breast Cancer
  • Cancer
  • Department Of Defense
  • Dielectric Polymers
  • Diseases And Disorders
  • Education
  • Electronic Mail
  • Information Operations
  • Macromolecules
  • Materials
  • Molecules
  • Neoplasms
  • Polyethylene Glycols

Readers

  • Molecular and Cellular Biochemistry
  • Oncology and Biomarker-Based Cancer Detection.
  • Trauma Surgery or Emergency Medicine.