Phage Langmuir-Blodgett Films for Biosensing Applications

Abstract

The microstructure of novel Langmuir-Blodgett (LB) monolayer films prepared from lytic bacteriophage as a biorecognition coating for methicillin-resistant Staphylococcus aureus (MRSA) bacterial biosensors was characterized using scanning imaging ellipsometry (SIE) and scanning electron microscopy (SEM). High lateral resolution SIE appeared to be ideal for the non-destructive analysis of ultra-thin organic LB films and complementary to SEM in comparison to other invasive techniques including atomic force microscopy, scanning tunneling microscopy, scanning force microscopy and X-ray diffraction. Field emission SEM permitted visual examination of monolayer structure. SIE allowed film thickness analysis, 3D mapping and monolayer surface imaging, and charged-coupled device (CCD) biosensing of MRSA.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2007
Accession Number
ADA521471

Entities

People

  • Eric Olsen
  • Iryna Sorokulova
  • Rajesh Guntupalli
  • Robert Long
  • William Neely

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Bacteriophages
  • Biosensors
  • Chemistry
  • Electron Microscopy
  • Fatty Acids
  • Field Emission
  • Films
  • Langmuir Blodgett Films
  • Microscopy
  • Monomolecular Films
  • Scanning
  • Scanning Electron Microscopy
  • Staphylococcus Aureus
  • Stearic Acid
  • Thickness
  • Three Dimensional
  • X-Ray Diffraction

Readers

  • Image Processing and Computer Vision.
  • Microbial Pathology
  • Thin Film Deposition Science.

Technology Areas

  • Biotechnology
  • Microelectronics
  • Microelectronics - Graphene