Human Neural Cell-Based Biosensor

Abstract

Human neural progenitors have a strong potential for use as cell-based biosensors for environmental toxins. In this report, we demonstrate that we have (1) optimized methods to genetically modify hNP1TM cells, (2) devised strategies for enriching the neural progenitor cell population using cell surface markers, and (3) developed methods for directed dopaminergic differentiation using defined medium conditions - all towards the goal of accelerating neuronal differentiation for biosensor development. Moreover, we have begun an exploration of fluorescence-based assays as a new direction for `sensor element' development, using neurite outgrowth as a test platform for screening neurotoxicants. Such exploration of alternate `sensor elements' beyond microelectrode arrays will broaden the utility of a human neural cell-based biosensor.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jun 11, 2010
Accession Number
ADA522666

Entities

People

  • Allan Powe
  • Jamie Chilton
  • Steven L. Stice

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Brain
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Cellular Structures
  • Culture Techniques
  • Department Of Defense
  • Electronic Mail
  • Growth Factors
  • Military Research
  • Nervous System
  • Neural Networks
  • Neurons
  • Proteins
  • Stem Cells
  • Transcription Factors
  • Tumor Cell Line

Readers

  • Neuroscience
  • Oncology and Biomarker-Based Cancer Detection.
  • Systems Analysis and Design

Technology Areas

  • Biotechnology