Humanized in vivo Model for Autoimmune Diabetes
Abstract
We previously reported (now published, 2009) that two human GAD65 555-567-responsive T cell receptors (TcRs) expressed in HLA DR0401 transgenic mice exhibit different mechanisms of self-tolerance. 164 TcR transgenic mice evoke tolerance by strong thymic negative selection and peripheral activation-induced cell death (AICD). 4.13 TcR transgenic mice select the TcR transgene near normal thymic cellularity levels and in the periphery result as a combination of IFN -secreting Th1 cells and IL-10-secreting Tr1 cells. Through crosses onto IL-10 knockout mice, we now have preliminary evidence that the regulatory cytokine IL-10 is maintaining an unactivated phenotype in 4.13 mice, which is in contrast to the self-antigenactivated phenotype observed in T cells from 164 mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2010
- Accession Number
- ADA523882
Entities
People
- Gerald T. Nepom
Organizations
- Benaroya Research Institute