Detection of 2-Photon Oxidation from a NIR Laser Using Confocal Microscopy

Abstract

Recent studies have determined that photochemical oxidation in cultured cells can be detected at peak irradiances as low as 8.5x10(8) W cm(-2) (87-fs pulse). Fluorescent dyes, such as CM-H(2)DCFDA, enable us to quantify the oxidation response of cells to mode-locked near-infrared (NIR) laser exposure. Using a modified confocal microscope, we characterize the time-dependent 2-photon induced fluorescence generated from a given NIR laser exposure. When cultured cells were then pre-loaded with antioxidants, ascorbic acid or N-acetyl-L-cysteine (NAC), they inhibit nonlinear oxidation with different efficiencies, providing insight regarding mechanisms of damage.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2006
Accession Number
ADA524140

Entities

People

  • Benjamin A. Rockwell
  • Kurt J. Schuster
  • Larry E. Estlack
  • Michael L. Denton

Organizations

  • Air Force Research Laboratory

Tags

Communities of Interest

  • Biomedical
  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Air Force
  • Antioxidants
  • Cells
  • Confocal Microscopy
  • Department Of Defense
  • Fluorescence
  • Fluorescent Dyes
  • Inhibitors
  • Laser Beams
  • Laser Pulses
  • Laser Safety
  • Lasers
  • Microscopes
  • Near Infrared Radiation
  • Optics
  • Vitamin C
  • Waveplates

Readers

  • Analytical Chemistry
  • Cellular and Molecular Pathways of Apoptosis.
  • Optical Physics and Photonics.

Technology Areas

  • Directed Energy