Interfering Breast Cancer Metastases by Blocking NGAL Function
Abstract
The first aim of this proposal is to identify small molecules that block NGAL function, a key molecule that our preliminary data suggested to be important in breast cancer and metastases. A high throughput screen was proposed to address this aim and currently we have constructed the essential reagents. The second goal is to examine the molecular mechanisms underlying NGAL function in breast tumorigenesis. In this regard, we have finished the mouse experiment depicting the role of NGAL in mammary tumor formation and the paper is in press in Cancer Research. In this publication, we found that ablation of mouse homologue of NGAL (mLCN2) significantly delayed the formation of mammary tumors in MMTV-ErbB2 (V664E) transgenic mice. We observed a much reduced tumor weight and delayed lung metastases in mLCN2 knockout background. Importantly, an affinity purified antibody against mLCN2 largely blocks the lung metastasis in the established 4T1 induced mammary tumors, suggesting the possibility of using a small molecule to inhibit NGAL function for breast cancer treatment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2009
- Accession Number
- ADA524471
Entities
People
- Ralph B. Arlinghaus
Organizations
- The University of Texas MD Anderson Cancer Center