Identification of Estrogen Receptor Beta Binding Sites in the Human Genomes
Abstract
With the recent discovery of ERbeta, the role of estrogens in the prostate becomes more complex and interesting. We hypothesize that ERbeta plays an essential role in the development and progression of prostate cancer. The major goal of this study is to use ChIP-seq to map ERbeta genome-wide binding sites and identify its target genes. We have successfully generated three cell lines stably expressing ERbeta as a FLAG-tagged fusion protein which are used to study the genomic functions of this steroid receptor. So far, the mapping of ERbeta was optimized in MCF7-C4-12-ERbeta. This provided the optimal conditions for our next step, which is mapping ERbeta binding sites in a prostate cancer cell line stably expressing this protein. Within the human genome, where ERbeta binds to and how it functions there will provide a better understanding of ERbeta genomic role in the normal and cancerous prostate. Such finding will undoubtedly provide considerable contribution to our knowledge of the complex interactions between androgen receptor, estrogen receptors, and possibly other proteins involved in the development of the prostate.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2010
- Accession Number
- ADA524511
Entities
People
- Thien Le
Organizations
- University of Chicago