Modulation of Beta-catenin activity with PKD1 in Prostate Cancer
Abstract
During the 2009-10 funding cycle we have made significant progress on our project. We have published six papers and seven abstracts. In addition to publications, we have obtained grants from the state (Governor's 2010 initiative), NIH (NCI RO1) and pharmaceutical industries (Merck Pharmaceuticals, Investigator Initiated Grant). In this study cycle, we attempted to isolate stable C4-2 cell lines transfected with PKD1 mutant constructs in order to identify the domains involved in interaction and modulation of Beta-catenin activity. While we had difficult time generating and isolating stably transfected cell lines, we used transiently transfected C4-2 cell lines to investigate the effect of PKD mutants on the various aspects of cellular functions including cell proliferation, colony formation and motility. The results from these experiments are presented below and provide promising results for further investigation. However, due to the low efficiency of transient transfection of C4-2 cells, we have decided to generate stably transfected C4-2 cell lines using retro-viral mediated transfection system to obtain reliable results. The retroviral system is a well established technique that yields much higher efficiency of generation of stable transfect compared to cationic-lipid mediated transfection. We, in collaboration with Dr. Chauhan, have also developed a xenograft mouse model system with the highly metastatic C4-2 cell lines. We have also discovered a new method of PKD1 activation by a dietary compound curcumin, results were presented at the AACR 2010 meeting. Our work related to curcumin pretreatment strategy of cancer cells induces chemo/radiosensitivity in cancer cells has been published. Based on clinical implications of this novel strategy, this paper was selected for press release.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2010
- Accession Number
- ADA524563
Entities
People
- Meena Jaggi
- Subhash C. Chauhan